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vivact 40 microct system  (SCANCO USA INC)

 
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    SCANCO USA INC vivact 40 microct system
    Vivact 40 Microct System, supplied by SCANCO USA INC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/microct+vivact+40/microct+vivact+40/pmc12052679-127-1-5
    Average 90 stars, based on 1 article reviews
    vivact 40 microct system - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Ex Vivo:

    Article Title: Ablation of Proliferating Osteoblast Lineage Cells After Fracture Leads to Atrophic Nonunion in a Mouse Model.
    Article Snippet: For each scan, the animal was anesthetized (1-3% isoflurane gas) and both femurs were scanned simultaneously using microCT (VivaCT 40, Scanco Medical AG, Switzerland; 15 μm voxel size, 70 kV, 114 μA, and 300 ms integration time).

    Article Title: Targeted clearance of p21- but not p16-positive senescent cells prevents radiation-induced osteoporosis and increased marrow adiposity.
    Article Snippet: 1Department of Physiology and Biomedical Engineering, Mayo Clinic, Rochester, Minnesota, USA 2Robert and Arlene Kogod Center on Aging, Mayo Clinic, Rochester, Minnesota, USA 3Division of Endocrinology, Mayo Clinic, Rochester, Minnesota, USA 4Department of Physical Medicine and Rehabilitation, Mayo Clinic, Rochester, Minnesota, USA 5Institute on the Biology of Aging and Metabolism, Department of Biochemistry, Molecular Biology and Biophysics, University of Minnesota, Minneapolis, Minnesota, USA 6Department of Pathology and Laboratory Medicine, University of Texas Health Science Center, San Antonio, Texas, USA

    Article Title: Proliferation of Early and Mature Osteoblasts Is Required for Bone Fracture Healing in Mice
    Article Snippet: Femurs were scanned ex vivo using microCT (VivaCT 40, Scanco, 10.5μm voxel size, 55kV, 145μA, 300ms integration time).

    Article Title: The development of a mouse model to investigate the formation of heterotopic ossification.
    Article Snippet: On day 7 and 14 after operation, all the mice were anaesthetized with isoflurane (1%–3%) or sacrificed for MicroCT (vivaCT 40; Scanco Medical, Bruttisellen, Switzerland) to detect HO formation.

    Article Title: Skeletal stem cell fate defects caused by Pdgfrb activating mutation.
    Article Snippet: For microCT, tibias were harvested and stored in 70% ethanol at 4°C, then analyzed to measure bone volume (mm3), total volume (mm3), mean/density (mg HA/ccm), trabecular numbers (1/mm), trabecular thickness (mm) and trabecular separation (mm) with a VivaCT 40 microCT (Scanco Medical) at an X-ray tube voltage of 70 kVp and an X-ray current of 114 μA for 2- to 3-week-old bones, or 55 kVp and 85 μA for older bones.

    Article Title: Ablation of Proliferating Osteoblast Lineage Cells After Fracture Leads to Atrophic Nonunion in a Mouse Model.
    Article Snippet: Femurs were scanned using microCT (VivaCT 40, Scanco Medical AG, Switzerland; 10.5 μm voxel size, 55 kV, 145 μA, 300 ms integration time).

    Article Title: VEGFA from osteoblasts is not required for lamellar bone formation following tibial loading.
    Article Snippet: Bones were scanned by microCT (vivaCT 40, Scanco Medical AG, 10.5 μm, 1000 projections, 300 ms, 70 kVp, 114 μA, high resolution).

    Article Title: In vivo microCT-based time-lapse morphometry reveals anatomical site-specific differences in bone (re)modeling serving as baseline parameters to detect early pathological events.
    Article Snippet: The hind limbs of the pilot tumor mouse (n = 1) were imaged in vivo with microCT (vivaCT 40, Scanco Medical, Switzerland).

    Control:

    Article Title: Ablation of Proliferating Osteoblast Lineage Cells After Fracture Leads to Atrophic Nonunion in a Mouse Model.
    Article Snippet: For each scan, the animal was anesthetized (1-3% isoflurane gas) and both femurs were scanned simultaneously using microCT (VivaCT 40, Scanco Medical AG, Switzerland; 15 μm voxel size, 70 kV, 114 μA, and 300 ms integration time).

    Article Title: Targeted clearance of p21- but not p16-positive senescent cells prevents radiation-induced osteoporosis and increased marrow adiposity.
    Article Snippet: 1Department of Physiology and Biomedical Engineering, Mayo Clinic, Rochester, Minnesota, USA 2Robert and Arlene Kogod Center on Aging, Mayo Clinic, Rochester, Minnesota, USA 3Division of Endocrinology, Mayo Clinic, Rochester, Minnesota, USA 4Department of Physical Medicine and Rehabilitation, Mayo Clinic, Rochester, Minnesota, USA 5Institute on the Biology of Aging and Metabolism, Department of Biochemistry, Molecular Biology and Biophysics, University of Minnesota, Minneapolis, Minnesota, USA 6Department of Pathology and Laboratory Medicine, University of Texas Health Science Center, San Antonio, Texas, USA

    Article Title: Proliferation of Early and Mature Osteoblasts Is Required for Bone Fracture Healing in Mice
    Article Snippet: Femurs were scanned ex vivo using microCT (VivaCT 40, Scanco, 10.5μm voxel size, 55kV, 145μA, 300ms integration time).

    Article Title: The development of a mouse model to investigate the formation of heterotopic ossification.
    Article Snippet: On day 7 and 14 after operation, all the mice were anaesthetized with isoflurane (1%–3%) or sacrificed for MicroCT (vivaCT 40; Scanco Medical, Bruttisellen, Switzerland) to detect HO formation.

    Article Title: Skeletal stem cell fate defects caused by Pdgfrb activating mutation.
    Article Snippet: For microCT, tibias were harvested and stored in 70% ethanol at 4°C, then analyzed to measure bone volume (mm3), total volume (mm3), mean/density (mg HA/ccm), trabecular numbers (1/mm), trabecular thickness (mm) and trabecular separation (mm) with a VivaCT 40 microCT (Scanco Medical) at an X-ray tube voltage of 70 kVp and an X-ray current of 114 μA for 2- to 3-week-old bones, or 55 kVp and 85 μA for older bones.

    Article Title: Ablation of Proliferating Osteoblast Lineage Cells After Fracture Leads to Atrophic Nonunion in a Mouse Model.
    Article Snippet: Femurs were scanned using microCT (VivaCT 40, Scanco Medical AG, Switzerland; 10.5 μm voxel size, 55 kV, 145 μA, 300 ms integration time).

    Article Title: VEGFA from osteoblasts is not required for lamellar bone formation following tibial loading.
    Article Snippet: Bones were scanned by microCT (vivaCT 40, Scanco Medical AG, 10.5 μm, 1000 projections, 300 ms, 70 kVp, 114 μA, high resolution).

    Article Title: In vivo microCT-based time-lapse morphometry reveals anatomical site-specific differences in bone (re)modeling serving as baseline parameters to detect early pathological events.
    Article Snippet: The hind limbs of the pilot tumor mouse (n = 1) were imaged in vivo with microCT (vivaCT 40, Scanco Medical, Switzerland).

    Staining:

    Article Title: Ablation of Proliferating Osteoblast Lineage Cells After Fracture Leads to Atrophic Nonunion in a Mouse Model.
    Article Snippet: For each scan, the animal was anesthetized (1-3% isoflurane gas) and both femurs were scanned simultaneously using microCT (VivaCT 40, Scanco Medical AG, Switzerland; 15 μm voxel size, 70 kV, 114 μA, and 300 ms integration time).

    Article Title: Targeted clearance of p21- but not p16-positive senescent cells prevents radiation-induced osteoporosis and increased marrow adiposity.
    Article Snippet: 1Department of Physiology and Biomedical Engineering, Mayo Clinic, Rochester, Minnesota, USA 2Robert and Arlene Kogod Center on Aging, Mayo Clinic, Rochester, Minnesota, USA 3Division of Endocrinology, Mayo Clinic, Rochester, Minnesota, USA 4Department of Physical Medicine and Rehabilitation, Mayo Clinic, Rochester, Minnesota, USA 5Institute on the Biology of Aging and Metabolism, Department of Biochemistry, Molecular Biology and Biophysics, University of Minnesota, Minneapolis, Minnesota, USA 6Department of Pathology and Laboratory Medicine, University of Texas Health Science Center, San Antonio, Texas, USA

    Article Title: Proliferation of Early and Mature Osteoblasts Is Required for Bone Fracture Healing in Mice
    Article Snippet: Femurs were scanned ex vivo using microCT (VivaCT 40, Scanco, 10.5μm voxel size, 55kV, 145μA, 300ms integration time).

    Article Title: The development of a mouse model to investigate the formation of heterotopic ossification.
    Article Snippet: On day 7 and 14 after operation, all the mice were anaesthetized with isoflurane (1%–3%) or sacrificed for MicroCT (vivaCT 40; Scanco Medical, Bruttisellen, Switzerland) to detect HO formation.

    Article Title: Skeletal stem cell fate defects caused by Pdgfrb activating mutation.
    Article Snippet: For microCT, tibias were harvested and stored in 70% ethanol at 4°C, then analyzed to measure bone volume (mm3), total volume (mm3), mean/density (mg HA/ccm), trabecular numbers (1/mm), trabecular thickness (mm) and trabecular separation (mm) with a VivaCT 40 microCT (Scanco Medical) at an X-ray tube voltage of 70 kVp and an X-ray current of 114 μA for 2- to 3-week-old bones, or 55 kVp and 85 μA for older bones.

    Article Title: Ablation of Proliferating Osteoblast Lineage Cells After Fracture Leads to Atrophic Nonunion in a Mouse Model.
    Article Snippet: Femurs were scanned using microCT (VivaCT 40, Scanco Medical AG, Switzerland; 10.5 μm voxel size, 55 kV, 145 μA, 300 ms integration time).

    Article Title: VEGFA from osteoblasts is not required for lamellar bone formation following tibial loading.
    Article Snippet: Bones were scanned by microCT (vivaCT 40, Scanco Medical AG, 10.5 μm, 1000 projections, 300 ms, 70 kVp, 114 μA, high resolution).

    Article Title: In vivo microCT-based time-lapse morphometry reveals anatomical site-specific differences in bone (re)modeling serving as baseline parameters to detect early pathological events.
    Article Snippet: The hind limbs of the pilot tumor mouse (n = 1) were imaged in vivo with microCT (vivaCT 40, Scanco Medical, Switzerland).

    Isolation:

    Article Title: Ablation of Proliferating Osteoblast Lineage Cells After Fracture Leads to Atrophic Nonunion in a Mouse Model.
    Article Snippet: For each scan, the animal was anesthetized (1-3% isoflurane gas) and both femurs were scanned simultaneously using microCT (VivaCT 40, Scanco Medical AG, Switzerland; 15 μm voxel size, 70 kV, 114 μA, and 300 ms integration time).

    Article Title: Targeted clearance of p21- but not p16-positive senescent cells prevents radiation-induced osteoporosis and increased marrow adiposity.
    Article Snippet: 1Department of Physiology and Biomedical Engineering, Mayo Clinic, Rochester, Minnesota, USA 2Robert and Arlene Kogod Center on Aging, Mayo Clinic, Rochester, Minnesota, USA 3Division of Endocrinology, Mayo Clinic, Rochester, Minnesota, USA 4Department of Physical Medicine and Rehabilitation, Mayo Clinic, Rochester, Minnesota, USA 5Institute on the Biology of Aging and Metabolism, Department of Biochemistry, Molecular Biology and Biophysics, University of Minnesota, Minneapolis, Minnesota, USA 6Department of Pathology and Laboratory Medicine, University of Texas Health Science Center, San Antonio, Texas, USA

    Article Title: Proliferation of Early and Mature Osteoblasts Is Required for Bone Fracture Healing in Mice
    Article Snippet: Femurs were scanned ex vivo using microCT (VivaCT 40, Scanco, 10.5μm voxel size, 55kV, 145μA, 300ms integration time).

    Article Title: The development of a mouse model to investigate the formation of heterotopic ossification.
    Article Snippet: On day 7 and 14 after operation, all the mice were anaesthetized with isoflurane (1%–3%) or sacrificed for MicroCT (vivaCT 40; Scanco Medical, Bruttisellen, Switzerland) to detect HO formation.

    Article Title: Skeletal stem cell fate defects caused by Pdgfrb activating mutation.
    Article Snippet: For microCT, tibias were harvested and stored in 70% ethanol at 4°C, then analyzed to measure bone volume (mm3), total volume (mm3), mean/density (mg HA/ccm), trabecular numbers (1/mm), trabecular thickness (mm) and trabecular separation (mm) with a VivaCT 40 microCT (Scanco Medical) at an X-ray tube voltage of 70 kVp and an X-ray current of 114 μA for 2- to 3-week-old bones, or 55 kVp and 85 μA for older bones.

    Article Title: Ablation of Proliferating Osteoblast Lineage Cells After Fracture Leads to Atrophic Nonunion in a Mouse Model.
    Article Snippet: Femurs were scanned using microCT (VivaCT 40, Scanco Medical AG, Switzerland; 10.5 μm voxel size, 55 kV, 145 μA, 300 ms integration time).

    Article Title: VEGFA from osteoblasts is not required for lamellar bone formation following tibial loading.
    Article Snippet: Bones were scanned by microCT (vivaCT 40, Scanco Medical AG, 10.5 μm, 1000 projections, 300 ms, 70 kVp, 114 μA, high resolution).

    Article Title: In vivo microCT-based time-lapse morphometry reveals anatomical site-specific differences in bone (re)modeling serving as baseline parameters to detect early pathological events.
    Article Snippet: The hind limbs of the pilot tumor mouse (n = 1) were imaged in vivo with microCT (vivaCT 40, Scanco Medical, Switzerland).

    In Silico:

    Article Title: Ablation of Proliferating Osteoblast Lineage Cells After Fracture Leads to Atrophic Nonunion in a Mouse Model.
    Article Snippet: For each scan, the animal was anesthetized (1-3% isoflurane gas) and both femurs were scanned simultaneously using microCT (VivaCT 40, Scanco Medical AG, Switzerland; 15 μm voxel size, 70 kV, 114 μA, and 300 ms integration time).

    Article Title: Targeted clearance of p21- but not p16-positive senescent cells prevents radiation-induced osteoporosis and increased marrow adiposity.
    Article Snippet: 1Department of Physiology and Biomedical Engineering, Mayo Clinic, Rochester, Minnesota, USA 2Robert and Arlene Kogod Center on Aging, Mayo Clinic, Rochester, Minnesota, USA 3Division of Endocrinology, Mayo Clinic, Rochester, Minnesota, USA 4Department of Physical Medicine and Rehabilitation, Mayo Clinic, Rochester, Minnesota, USA 5Institute on the Biology of Aging and Metabolism, Department of Biochemistry, Molecular Biology and Biophysics, University of Minnesota, Minneapolis, Minnesota, USA 6Department of Pathology and Laboratory Medicine, University of Texas Health Science Center, San Antonio, Texas, USA

    Article Title: Proliferation of Early and Mature Osteoblasts Is Required for Bone Fracture Healing in Mice
    Article Snippet: Femurs were scanned ex vivo using microCT (VivaCT 40, Scanco, 10.5μm voxel size, 55kV, 145μA, 300ms integration time).

    Article Title: The development of a mouse model to investigate the formation of heterotopic ossification.
    Article Snippet: On day 7 and 14 after operation, all the mice were anaesthetized with isoflurane (1%–3%) or sacrificed for MicroCT (vivaCT 40; Scanco Medical, Bruttisellen, Switzerland) to detect HO formation.

    Article Title: Skeletal stem cell fate defects caused by Pdgfrb activating mutation.
    Article Snippet: For microCT, tibias were harvested and stored in 70% ethanol at 4°C, then analyzed to measure bone volume (mm3), total volume (mm3), mean/density (mg HA/ccm), trabecular numbers (1/mm), trabecular thickness (mm) and trabecular separation (mm) with a VivaCT 40 microCT (Scanco Medical) at an X-ray tube voltage of 70 kVp and an X-ray current of 114 μA for 2- to 3-week-old bones, or 55 kVp and 85 μA for older bones.

    Article Title: Ablation of Proliferating Osteoblast Lineage Cells After Fracture Leads to Atrophic Nonunion in a Mouse Model.
    Article Snippet: Femurs were scanned using microCT (VivaCT 40, Scanco Medical AG, Switzerland; 10.5 μm voxel size, 55 kV, 145 μA, 300 ms integration time).

    Article Title: VEGFA from osteoblasts is not required for lamellar bone formation following tibial loading.
    Article Snippet: Bones were scanned by microCT (vivaCT 40, Scanco Medical AG, 10.5 μm, 1000 projections, 300 ms, 70 kVp, 114 μA, high resolution).

    Article Title: In vivo microCT-based time-lapse morphometry reveals anatomical site-specific differences in bone (re)modeling serving as baseline parameters to detect early pathological events.
    Article Snippet: The hind limbs of the pilot tumor mouse (n = 1) were imaged in vivo with microCT (vivaCT 40, Scanco Medical, Switzerland).

    Expressing:

    Article Title: Ablation of Proliferating Osteoblast Lineage Cells After Fracture Leads to Atrophic Nonunion in a Mouse Model.
    Article Snippet: For each scan, the animal was anesthetized (1-3% isoflurane gas) and both femurs were scanned simultaneously using microCT (VivaCT 40, Scanco Medical AG, Switzerland; 15 μm voxel size, 70 kV, 114 μA, and 300 ms integration time).

    Article Title: Targeted clearance of p21- but not p16-positive senescent cells prevents radiation-induced osteoporosis and increased marrow adiposity.
    Article Snippet: 1Department of Physiology and Biomedical Engineering, Mayo Clinic, Rochester, Minnesota, USA 2Robert and Arlene Kogod Center on Aging, Mayo Clinic, Rochester, Minnesota, USA 3Division of Endocrinology, Mayo Clinic, Rochester, Minnesota, USA 4Department of Physical Medicine and Rehabilitation, Mayo Clinic, Rochester, Minnesota, USA 5Institute on the Biology of Aging and Metabolism, Department of Biochemistry, Molecular Biology and Biophysics, University of Minnesota, Minneapolis, Minnesota, USA 6Department of Pathology and Laboratory Medicine, University of Texas Health Science Center, San Antonio, Texas, USA

    Article Title: Proliferation of Early and Mature Osteoblasts Is Required for Bone Fracture Healing in Mice
    Article Snippet: Femurs were scanned ex vivo using microCT (VivaCT 40, Scanco, 10.5μm voxel size, 55kV, 145μA, 300ms integration time).

    Article Title: The development of a mouse model to investigate the formation of heterotopic ossification.
    Article Snippet: On day 7 and 14 after operation, all the mice were anaesthetized with isoflurane (1%–3%) or sacrificed for MicroCT (vivaCT 40; Scanco Medical, Bruttisellen, Switzerland) to detect HO formation.

    Article Title: Skeletal stem cell fate defects caused by Pdgfrb activating mutation.
    Article Snippet: For microCT, tibias were harvested and stored in 70% ethanol at 4°C, then analyzed to measure bone volume (mm3), total volume (mm3), mean/density (mg HA/ccm), trabecular numbers (1/mm), trabecular thickness (mm) and trabecular separation (mm) with a VivaCT 40 microCT (Scanco Medical) at an X-ray tube voltage of 70 kVp and an X-ray current of 114 μA for 2- to 3-week-old bones, or 55 kVp and 85 μA for older bones.

    Article Title: Ablation of Proliferating Osteoblast Lineage Cells After Fracture Leads to Atrophic Nonunion in a Mouse Model.
    Article Snippet: Femurs were scanned using microCT (VivaCT 40, Scanco Medical AG, Switzerland; 10.5 μm voxel size, 55 kV, 145 μA, 300 ms integration time).

    Article Title: VEGFA from osteoblasts is not required for lamellar bone formation following tibial loading.
    Article Snippet: Bones were scanned by microCT (vivaCT 40, Scanco Medical AG, 10.5 μm, 1000 projections, 300 ms, 70 kVp, 114 μA, high resolution).

    Article Title: In vivo microCT-based time-lapse morphometry reveals anatomical site-specific differences in bone (re)modeling serving as baseline parameters to detect early pathological events.
    Article Snippet: The hind limbs of the pilot tumor mouse (n = 1) were imaged in vivo with microCT (vivaCT 40, Scanco Medical, Switzerland).

    Two Tailed Test:

    Article Title: Ablation of Proliferating Osteoblast Lineage Cells After Fracture Leads to Atrophic Nonunion in a Mouse Model.
    Article Snippet: For each scan, the animal was anesthetized (1-3% isoflurane gas) and both femurs were scanned simultaneously using microCT (VivaCT 40, Scanco Medical AG, Switzerland; 15 μm voxel size, 70 kV, 114 μA, and 300 ms integration time).

    Article Title: Targeted clearance of p21- but not p16-positive senescent cells prevents radiation-induced osteoporosis and increased marrow adiposity.
    Article Snippet: 1Department of Physiology and Biomedical Engineering, Mayo Clinic, Rochester, Minnesota, USA 2Robert and Arlene Kogod Center on Aging, Mayo Clinic, Rochester, Minnesota, USA 3Division of Endocrinology, Mayo Clinic, Rochester, Minnesota, USA 4Department of Physical Medicine and Rehabilitation, Mayo Clinic, Rochester, Minnesota, USA 5Institute on the Biology of Aging and Metabolism, Department of Biochemistry, Molecular Biology and Biophysics, University of Minnesota, Minneapolis, Minnesota, USA 6Department of Pathology and Laboratory Medicine, University of Texas Health Science Center, San Antonio, Texas, USA

    Article Title: Proliferation of Early and Mature Osteoblasts Is Required for Bone Fracture Healing in Mice
    Article Snippet: Femurs were scanned ex vivo using microCT (VivaCT 40, Scanco, 10.5μm voxel size, 55kV, 145μA, 300ms integration time).

    Article Title: The development of a mouse model to investigate the formation of heterotopic ossification.
    Article Snippet: On day 7 and 14 after operation, all the mice were anaesthetized with isoflurane (1%–3%) or sacrificed for MicroCT (vivaCT 40; Scanco Medical, Bruttisellen, Switzerland) to detect HO formation.

    Article Title: Skeletal stem cell fate defects caused by Pdgfrb activating mutation.
    Article Snippet: For microCT, tibias were harvested and stored in 70% ethanol at 4°C, then analyzed to measure bone volume (mm3), total volume (mm3), mean/density (mg HA/ccm), trabecular numbers (1/mm), trabecular thickness (mm) and trabecular separation (mm) with a VivaCT 40 microCT (Scanco Medical) at an X-ray tube voltage of 70 kVp and an X-ray current of 114 μA for 2- to 3-week-old bones, or 55 kVp and 85 μA for older bones.

    Article Title: Ablation of Proliferating Osteoblast Lineage Cells After Fracture Leads to Atrophic Nonunion in a Mouse Model.
    Article Snippet: Femurs were scanned using microCT (VivaCT 40, Scanco Medical AG, Switzerland; 10.5 μm voxel size, 55 kV, 145 μA, 300 ms integration time).

    Article Title: VEGFA from osteoblasts is not required for lamellar bone formation following tibial loading.
    Article Snippet: Bones were scanned by microCT (vivaCT 40, Scanco Medical AG, 10.5 μm, 1000 projections, 300 ms, 70 kVp, 114 μA, high resolution).

    Article Title: In vivo microCT-based time-lapse morphometry reveals anatomical site-specific differences in bone (re)modeling serving as baseline parameters to detect early pathological events.
    Article Snippet: The hind limbs of the pilot tumor mouse (n = 1) were imaged in vivo with microCT (vivaCT 40, Scanco Medical, Switzerland).

    Blocking Assay:

    Article Title: Ablation of Proliferating Osteoblast Lineage Cells After Fracture Leads to Atrophic Nonunion in a Mouse Model.
    Article Snippet: For each scan, the animal was anesthetized (1-3% isoflurane gas) and both femurs were scanned simultaneously using microCT (VivaCT 40, Scanco Medical AG, Switzerland; 15 μm voxel size, 70 kV, 114 μA, and 300 ms integration time).

    Article Title: Targeted clearance of p21- but not p16-positive senescent cells prevents radiation-induced osteoporosis and increased marrow adiposity.
    Article Snippet: 1Department of Physiology and Biomedical Engineering, Mayo Clinic, Rochester, Minnesota, USA 2Robert and Arlene Kogod Center on Aging, Mayo Clinic, Rochester, Minnesota, USA 3Division of Endocrinology, Mayo Clinic, Rochester, Minnesota, USA 4Department of Physical Medicine and Rehabilitation, Mayo Clinic, Rochester, Minnesota, USA 5Institute on the Biology of Aging and Metabolism, Department of Biochemistry, Molecular Biology and Biophysics, University of Minnesota, Minneapolis, Minnesota, USA 6Department of Pathology and Laboratory Medicine, University of Texas Health Science Center, San Antonio, Texas, USA

    Article Title: Proliferation of Early and Mature Osteoblasts Is Required for Bone Fracture Healing in Mice
    Article Snippet: Femurs were scanned ex vivo using microCT (VivaCT 40, Scanco, 10.5μm voxel size, 55kV, 145μA, 300ms integration time).

    Article Title: The development of a mouse model to investigate the formation of heterotopic ossification.
    Article Snippet: On day 7 and 14 after operation, all the mice were anaesthetized with isoflurane (1%–3%) or sacrificed for MicroCT (vivaCT 40; Scanco Medical, Bruttisellen, Switzerland) to detect HO formation.

    Article Title: Skeletal stem cell fate defects caused by Pdgfrb activating mutation.
    Article Snippet: For microCT, tibias were harvested and stored in 70% ethanol at 4°C, then analyzed to measure bone volume (mm3), total volume (mm3), mean/density (mg HA/ccm), trabecular numbers (1/mm), trabecular thickness (mm) and trabecular separation (mm) with a VivaCT 40 microCT (Scanco Medical) at an X-ray tube voltage of 70 kVp and an X-ray current of 114 μA for 2- to 3-week-old bones, or 55 kVp and 85 μA for older bones.

    Article Title: Ablation of Proliferating Osteoblast Lineage Cells After Fracture Leads to Atrophic Nonunion in a Mouse Model.
    Article Snippet: Femurs were scanned using microCT (VivaCT 40, Scanco Medical AG, Switzerland; 10.5 μm voxel size, 55 kV, 145 μA, 300 ms integration time).

    Article Title: VEGFA from osteoblasts is not required for lamellar bone formation following tibial loading.
    Article Snippet: Bones were scanned by microCT (vivaCT 40, Scanco Medical AG, 10.5 μm, 1000 projections, 300 ms, 70 kVp, 114 μA, high resolution).

    Article Title: In vivo microCT-based time-lapse morphometry reveals anatomical site-specific differences in bone (re)modeling serving as baseline parameters to detect early pathological events.
    Article Snippet: The hind limbs of the pilot tumor mouse (n = 1) were imaged in vivo with microCT (vivaCT 40, Scanco Medical, Switzerland).

    MANN-WHITNEY:

    Article Title: Ablation of Proliferating Osteoblast Lineage Cells After Fracture Leads to Atrophic Nonunion in a Mouse Model.
    Article Snippet: For each scan, the animal was anesthetized (1-3% isoflurane gas) and both femurs were scanned simultaneously using microCT (VivaCT 40, Scanco Medical AG, Switzerland; 15 μm voxel size, 70 kV, 114 μA, and 300 ms integration time).

    Article Title: Targeted clearance of p21- but not p16-positive senescent cells prevents radiation-induced osteoporosis and increased marrow adiposity.
    Article Snippet: 1Department of Physiology and Biomedical Engineering, Mayo Clinic, Rochester, Minnesota, USA 2Robert and Arlene Kogod Center on Aging, Mayo Clinic, Rochester, Minnesota, USA 3Division of Endocrinology, Mayo Clinic, Rochester, Minnesota, USA 4Department of Physical Medicine and Rehabilitation, Mayo Clinic, Rochester, Minnesota, USA 5Institute on the Biology of Aging and Metabolism, Department of Biochemistry, Molecular Biology and Biophysics, University of Minnesota, Minneapolis, Minnesota, USA 6Department of Pathology and Laboratory Medicine, University of Texas Health Science Center, San Antonio, Texas, USA

    Article Title: Proliferation of Early and Mature Osteoblasts Is Required for Bone Fracture Healing in Mice
    Article Snippet: Femurs were scanned ex vivo using microCT (VivaCT 40, Scanco, 10.5μm voxel size, 55kV, 145μA, 300ms integration time).

    Article Title: The development of a mouse model to investigate the formation of heterotopic ossification.
    Article Snippet: On day 7 and 14 after operation, all the mice were anaesthetized with isoflurane (1%–3%) or sacrificed for MicroCT (vivaCT 40; Scanco Medical, Bruttisellen, Switzerland) to detect HO formation.

    Article Title: Skeletal stem cell fate defects caused by Pdgfrb activating mutation.
    Article Snippet: For microCT, tibias were harvested and stored in 70% ethanol at 4°C, then analyzed to measure bone volume (mm3), total volume (mm3), mean/density (mg HA/ccm), trabecular numbers (1/mm), trabecular thickness (mm) and trabecular separation (mm) with a VivaCT 40 microCT (Scanco Medical) at an X-ray tube voltage of 70 kVp and an X-ray current of 114 μA for 2- to 3-week-old bones, or 55 kVp and 85 μA for older bones.

    Article Title: Ablation of Proliferating Osteoblast Lineage Cells After Fracture Leads to Atrophic Nonunion in a Mouse Model.
    Article Snippet: Femurs were scanned using microCT (VivaCT 40, Scanco Medical AG, Switzerland; 10.5 μm voxel size, 55 kV, 145 μA, 300 ms integration time).

    Article Title: VEGFA from osteoblasts is not required for lamellar bone formation following tibial loading.
    Article Snippet: Bones were scanned by microCT (vivaCT 40, Scanco Medical AG, 10.5 μm, 1000 projections, 300 ms, 70 kVp, 114 μA, high resolution).

    Article Title: In vivo microCT-based time-lapse morphometry reveals anatomical site-specific differences in bone (re)modeling serving as baseline parameters to detect early pathological events.
    Article Snippet: The hind limbs of the pilot tumor mouse (n = 1) were imaged in vivo with microCT (vivaCT 40, Scanco Medical, Switzerland).



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    (A) Femur fractures were created in 12-week-old wildtype control (CTL) and Col1-TK experimental (EXPT) mice, followed by ganciclovir (GCV) dosing for 3, 7, or 14 days (n=7-9). (B) (Left) Representative radiographs at 21 days post-fracture (DPF). (Right) Quantification of radiographic healing. (C) 3D <t>microCT</t> images at 21 DPF (scale bar, 1 mm). (D) Sagittal callus sections were stained with picrosirius red (PSR) to visualize collagen-rich bone and alcian blue (AB) for proteoglycan-rich cartilage, and callus composition was determined (scale bar=1 mm). (Graphs depict mean ± SD; individual data points shown [pink=female; blue=male]. Statistics: Chi-square test (B) or two-Way ANOVA with Tukey Post Hoc test (C,D); *p<0.05, **<0.01, ****<0.0001; ns: p>0.05)
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    (A) Femur fractures were created in 12-week-old wildtype control (CTL) and Col1-TK experimental (EXPT) mice, followed by ganciclovir (GCV) dosing for 3, 7, or 14 days (n=7-9). (B) (Left) Representative radiographs at 21 days post-fracture (DPF). (Right) Quantification of radiographic healing. (C) 3D <t>microCT</t> images at 21 DPF (scale bar, 1 mm). (D) Sagittal callus sections were stained with picrosirius red (PSR) to visualize collagen-rich bone and alcian blue (AB) for proteoglycan-rich cartilage, and callus composition was determined (scale bar=1 mm). (Graphs depict mean ± SD; individual data points shown [pink=female; blue=male]. Statistics: Chi-square test (B) or two-Way ANOVA with Tukey Post Hoc test (C,D); *p<0.05, **<0.01, ****<0.0001; ns: p>0.05)
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    Twelve-week old male Cre-negative (Cre-) and Cre-positive (Cre +) Bmpr1a fl/fl ;LysM-Cre mice remained euthyroid (CO) or were rendered hyperthyroid (T4) by adding 1.2 µg/mL L-thyroxine into their drinking water over 4 weeks. Using <t>microCT</t> analysis, ( a ) bone volume per total volume (BV/TV), ( b ) trabecular number (Tb.N), ( c ) trabecular separation (Tb.Sp), and ( d ) trabecular thickness (Tb.Th) were determined at the fourth lumbar vertebra (L4). e Representative 3D reconstructions of the trabecular compartment of L4. Additionally, ( f ) trabecular BV/TV and ( g ) cortical bone volume over total volume (Ct.BV/TV) were assessed at the femur. The maximal force (F max ) as an indicator of bone strength ( h ) at the L5 vertebra and ( i ) femur was tested by compression test and 3-point bending test, respectively. Serum concentrations of ( j ) bone formation marker P1NP and ( k ) bone resorption marker TRAP were analyzed by ELISA. Furthermore, ( l ) the bone formation rate per bone surface (BFR) and ( m ) osteoclast surface per bone surface (Oc.S/BS) were assessed at the L3 and L4 vertebra, respectively. In vitro, ( n ) expression of acid phosphatase 5, tartrate resistant ( Acp5 ), encoding for TRAP, in primary osteoclasts derived from Bmpr1a fl/fl ;LysM-Cre mice at day 7 of differentiation, with or w/o treatment with 100 nM T 3 (T3) over 48 h, was quantified using quantitative real-time PCR. Each dot indicates an individual mouse. In vivo: Cre-/CO: N = 9; Cre-/T4: N = 8; Cre +/CO: N = 9; Cre + /T4: N = 9; In vitro: Cre-/CO: N = 5; Cre-/T3: N = 6; Cre +/CO: N = 6; Cre +/T3: N = 6. The horizontal lines represent the mean +/- SD. Statistical analysis was performed by Two-way ANOVA and selected p values are shown within the graph.
    Micro Computed Tomography (Microct) Vivact 40, supplied by SCANCO USA INC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Twelve-week old male Cre-negative (Cre-) and Cre-positive (Cre +) Bmpr1a fl/fl ;LysM-Cre mice remained euthyroid (CO) or were rendered hyperthyroid (T4) by adding 1.2 µg/mL L-thyroxine into their drinking water over 4 weeks. Using <t>microCT</t> analysis, ( a ) bone volume per total volume (BV/TV), ( b ) trabecular number (Tb.N), ( c ) trabecular separation (Tb.Sp), and ( d ) trabecular thickness (Tb.Th) were determined at the fourth lumbar vertebra (L4). e Representative 3D reconstructions of the trabecular compartment of L4. Additionally, ( f ) trabecular BV/TV and ( g ) cortical bone volume over total volume (Ct.BV/TV) were assessed at the femur. The maximal force (F max ) as an indicator of bone strength ( h ) at the L5 vertebra and ( i ) femur was tested by compression test and 3-point bending test, respectively. Serum concentrations of ( j ) bone formation marker P1NP and ( k ) bone resorption marker TRAP were analyzed by ELISA. Furthermore, ( l ) the bone formation rate per bone surface (BFR) and ( m ) osteoclast surface per bone surface (Oc.S/BS) were assessed at the L3 and L4 vertebra, respectively. In vitro, ( n ) expression of acid phosphatase 5, tartrate resistant ( Acp5 ), encoding for TRAP, in primary osteoclasts derived from Bmpr1a fl/fl ;LysM-Cre mice at day 7 of differentiation, with or w/o treatment with 100 nM T 3 (T3) over 48 h, was quantified using quantitative real-time PCR. Each dot indicates an individual mouse. In vivo: Cre-/CO: N = 9; Cre-/T4: N = 8; Cre +/CO: N = 9; Cre + /T4: N = 9; In vitro: Cre-/CO: N = 5; Cre-/T3: N = 6; Cre +/CO: N = 6; Cre +/T3: N = 6. The horizontal lines represent the mean +/- SD. Statistical analysis was performed by Two-way ANOVA and selected p values are shown within the graph.
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    Twelve-week old male Cre-negative (Cre-) and Cre-positive (Cre +) Bmpr1a fl/fl ;LysM-Cre mice remained euthyroid (CO) or were rendered hyperthyroid (T4) by adding 1.2 µg/mL L-thyroxine into their drinking water over 4 weeks. Using <t>microCT</t> analysis, ( a ) bone volume per total volume (BV/TV), ( b ) trabecular number (Tb.N), ( c ) trabecular separation (Tb.Sp), and ( d ) trabecular thickness (Tb.Th) were determined at the fourth lumbar vertebra (L4). e Representative 3D reconstructions of the trabecular compartment of L4. Additionally, ( f ) trabecular BV/TV and ( g ) cortical bone volume over total volume (Ct.BV/TV) were assessed at the femur. The maximal force (F max ) as an indicator of bone strength ( h ) at the L5 vertebra and ( i ) femur was tested by compression test and 3-point bending test, respectively. Serum concentrations of ( j ) bone formation marker P1NP and ( k ) bone resorption marker TRAP were analyzed by ELISA. Furthermore, ( l ) the bone formation rate per bone surface (BFR) and ( m ) osteoclast surface per bone surface (Oc.S/BS) were assessed at the L3 and L4 vertebra, respectively. In vitro, ( n ) expression of acid phosphatase 5, tartrate resistant ( Acp5 ), encoding for TRAP, in primary osteoclasts derived from Bmpr1a fl/fl ;LysM-Cre mice at day 7 of differentiation, with or w/o treatment with 100 nM T 3 (T3) over 48 h, was quantified using quantitative real-time PCR. Each dot indicates an individual mouse. In vivo: Cre-/CO: N = 9; Cre-/T4: N = 8; Cre +/CO: N = 9; Cre + /T4: N = 9; In vitro: Cre-/CO: N = 5; Cre-/T3: N = 6; Cre +/CO: N = 6; Cre +/T3: N = 6. The horizontal lines represent the mean +/- SD. Statistical analysis was performed by Two-way ANOVA and selected p values are shown within the graph.
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    Twelve-week old male Cre-negative (Cre-) and Cre-positive (Cre +) Bmpr1a fl/fl ;LysM-Cre mice remained euthyroid (CO) or were rendered hyperthyroid (T4) by adding 1.2 µg/mL L-thyroxine into their drinking water over 4 weeks. Using <t>microCT</t> analysis, ( a ) bone volume per total volume (BV/TV), ( b ) trabecular number (Tb.N), ( c ) trabecular separation (Tb.Sp), and ( d ) trabecular thickness (Tb.Th) were determined at the fourth lumbar vertebra (L4). e Representative 3D reconstructions of the trabecular compartment of L4. Additionally, ( f ) trabecular BV/TV and ( g ) cortical bone volume over total volume (Ct.BV/TV) were assessed at the femur. The maximal force (F max ) as an indicator of bone strength ( h ) at the L5 vertebra and ( i ) femur was tested by compression test and 3-point bending test, respectively. Serum concentrations of ( j ) bone formation marker P1NP and ( k ) bone resorption marker TRAP were analyzed by ELISA. Furthermore, ( l ) the bone formation rate per bone surface (BFR) and ( m ) osteoclast surface per bone surface (Oc.S/BS) were assessed at the L3 and L4 vertebra, respectively. In vitro, ( n ) expression of acid phosphatase 5, tartrate resistant ( Acp5 ), encoding for TRAP, in primary osteoclasts derived from Bmpr1a fl/fl ;LysM-Cre mice at day 7 of differentiation, with or w/o treatment with 100 nM T 3 (T3) over 48 h, was quantified using quantitative real-time PCR. Each dot indicates an individual mouse. In vivo: Cre-/CO: N = 9; Cre-/T4: N = 8; Cre +/CO: N = 9; Cre + /T4: N = 9; In vitro: Cre-/CO: N = 5; Cre-/T3: N = 6; Cre +/CO: N = 6; Cre +/T3: N = 6. The horizontal lines represent the mean +/- SD. Statistical analysis was performed by Two-way ANOVA and selected p values are shown within the graph.
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    Image Search Results


    (A) Femur fractures were created in 12-week-old wildtype control (CTL) and Col1-TK experimental (EXPT) mice, followed by ganciclovir (GCV) dosing for 3, 7, or 14 days (n=7-9). (B) (Left) Representative radiographs at 21 days post-fracture (DPF). (Right) Quantification of radiographic healing. (C) 3D microCT images at 21 DPF (scale bar, 1 mm). (D) Sagittal callus sections were stained with picrosirius red (PSR) to visualize collagen-rich bone and alcian blue (AB) for proteoglycan-rich cartilage, and callus composition was determined (scale bar=1 mm). (Graphs depict mean ± SD; individual data points shown [pink=female; blue=male]. Statistics: Chi-square test (B) or two-Way ANOVA with Tukey Post Hoc test (C,D); *p<0.05, **<0.01, ****<0.0001; ns: p>0.05)

    Journal: bioRxiv

    Article Title: Proliferation of Early and Mature Osteoblasts Is Required for Bone Fracture Healing in Mice

    doi: 10.1101/2025.05.27.656371

    Figure Lengend Snippet: (A) Femur fractures were created in 12-week-old wildtype control (CTL) and Col1-TK experimental (EXPT) mice, followed by ganciclovir (GCV) dosing for 3, 7, or 14 days (n=7-9). (B) (Left) Representative radiographs at 21 days post-fracture (DPF). (Right) Quantification of radiographic healing. (C) 3D microCT images at 21 DPF (scale bar, 1 mm). (D) Sagittal callus sections were stained with picrosirius red (PSR) to visualize collagen-rich bone and alcian blue (AB) for proteoglycan-rich cartilage, and callus composition was determined (scale bar=1 mm). (Graphs depict mean ± SD; individual data points shown [pink=female; blue=male]. Statistics: Chi-square test (B) or two-Way ANOVA with Tukey Post Hoc test (C,D); *p<0.05, **<0.01, ****<0.0001; ns: p>0.05)

    Article Snippet: Femurs were scanned ex vivo using microCT (VivaCT 40, Scanco, 10.5μm voxel size, 55kV, 145μA, 300ms integration time).

    Techniques: Control, Staining

    (A) Radiographic scoring by dosing duration at 7, 14 and 21 days post-fracture (DPF) (n=7-9). (Score 2=neither cortex bridged; 6=both cortices bridged). (B) microCT parameters from the callus region at 21 DPF (includes callus + cortical bone). (Dashed lines indicate avg. value of contralateral intact femurs; shading denotes +/- SD). (C) Histological callus composition at 21 DPF was determined from sections stained by picrosirius red and alcian blue (PSRAB). Image is representative section from Col1-TK mouse treated for 7 days with GCV. (Cg: cartilage, Wb: woven bone, F: fibrous tissue, Ps: periosteum.) (Graphs B, C depict mean ± SD; statistical differences determined by Fisher’s Exact Test)

    Journal: bioRxiv

    Article Title: Proliferation of Early and Mature Osteoblasts Is Required for Bone Fracture Healing in Mice

    doi: 10.1101/2025.05.27.656371

    Figure Lengend Snippet: (A) Radiographic scoring by dosing duration at 7, 14 and 21 days post-fracture (DPF) (n=7-9). (Score 2=neither cortex bridged; 6=both cortices bridged). (B) microCT parameters from the callus region at 21 DPF (includes callus + cortical bone). (Dashed lines indicate avg. value of contralateral intact femurs; shading denotes +/- SD). (C) Histological callus composition at 21 DPF was determined from sections stained by picrosirius red and alcian blue (PSRAB). Image is representative section from Col1-TK mouse treated for 7 days with GCV. (Cg: cartilage, Wb: woven bone, F: fibrous tissue, Ps: periosteum.) (Graphs B, C depict mean ± SD; statistical differences determined by Fisher’s Exact Test)

    Article Snippet: Femurs were scanned ex vivo using microCT (VivaCT 40, Scanco, 10.5μm voxel size, 55kV, 145μA, 300ms integration time).

    Techniques: Staining

    (A) After femur fracture Col1-TK mice were treated with water (Control, n=3) or GCV (Exptl, n=3) for 10 days. Cells isolated from whole-fracture callus were subjected to scRNAseq. (B) UMAP plot of total callus cells with annotated cluster identities. (C) The number of cells from immune clusters on Day 5 (Clusters 2, 6, 7, 10, 11, 12, 14, 16) and Day 10 (Clusters 7, 10, 13, 15) normalized to total cell numbers. (D) Mesenchymal cells were selected in silico and re-clustered. (E) Expression of canonical genes for osteoblasts, chondrocytes, and hypertrophic chondrocytes displayed as violin plots and projected on UMAPS. (F) Mesenchymal cells were identified by treatment group, and the percent of cells in each cluster was quantified (n=3). (G) Callus bone volume was quantified by microCT (n=5-8). (H) Histological sections of callus at 10 DPF were analyzed to determine total callus area and callus tissue composition. (I) Representative images of TK IHC of control (Col1-TK + water) and exptl (Col1-TK + GCV). (Statistics: two-Way ANOVA with Holm-Sidak Post Hoc test (C, F) or two-tailed t-test (G, H). Scale bars: PSRAB = 1 mm, TK=1 mm.)

    Journal: bioRxiv

    Article Title: Proliferation of Early and Mature Osteoblasts Is Required for Bone Fracture Healing in Mice

    doi: 10.1101/2025.05.27.656371

    Figure Lengend Snippet: (A) After femur fracture Col1-TK mice were treated with water (Control, n=3) or GCV (Exptl, n=3) for 10 days. Cells isolated from whole-fracture callus were subjected to scRNAseq. (B) UMAP plot of total callus cells with annotated cluster identities. (C) The number of cells from immune clusters on Day 5 (Clusters 2, 6, 7, 10, 11, 12, 14, 16) and Day 10 (Clusters 7, 10, 13, 15) normalized to total cell numbers. (D) Mesenchymal cells were selected in silico and re-clustered. (E) Expression of canonical genes for osteoblasts, chondrocytes, and hypertrophic chondrocytes displayed as violin plots and projected on UMAPS. (F) Mesenchymal cells were identified by treatment group, and the percent of cells in each cluster was quantified (n=3). (G) Callus bone volume was quantified by microCT (n=5-8). (H) Histological sections of callus at 10 DPF were analyzed to determine total callus area and callus tissue composition. (I) Representative images of TK IHC of control (Col1-TK + water) and exptl (Col1-TK + GCV). (Statistics: two-Way ANOVA with Holm-Sidak Post Hoc test (C, F) or two-tailed t-test (G, H). Scale bars: PSRAB = 1 mm, TK=1 mm.)

    Article Snippet: Femurs were scanned ex vivo using microCT (VivaCT 40, Scanco, 10.5μm voxel size, 55kV, 145μA, 300ms integration time).

    Techniques: Control, Isolation, In Silico, Expressing, Two Tailed Test

    (A) Overlay of Prrx1 (mesenchymal cells), Pecam1 (endothelial cells), or Ptprc /CD45 (immune cells) of all cells 10 days post-fracture. (B) Top 5 DEGs for each cluster of all cells from 10 DPF. (C) UMAP colored by group (Control, Exptl). The percent of cells in each cluster by group was quantified (number of cells in cluster/total number of cells from that callus) for each replicate callus and plotted (n=3). (D) Number of Skeletal Stem and Progenitor Cells (SSPCs, defined as cells expressing Acta2 , Ly6a , Itgav , Thy1 , and Ctsk ) normalized to the total number of cells from that replicate callus. (E) Expression of myofibroblast genes ( Acta2 , Tagln2 , Actg1 , and Tpm2 ), nonspecific fibroblast genes ( Col1a1 , Col3a1 , and Postn ), and innate immune genes ( Isg15 , Ifit1 , Irf7 ) used to annotate mesenchymal cell clusters. (F) MicroCT analysis of fracture callus. (G) Histological analysis of callus composition. (Graphs depict mean±SD; statistical differences were determined by a two-Way ANOVA with Holm-Sidak Post-Hoc test.)

    Journal: bioRxiv

    Article Title: Proliferation of Early and Mature Osteoblasts Is Required for Bone Fracture Healing in Mice

    doi: 10.1101/2025.05.27.656371

    Figure Lengend Snippet: (A) Overlay of Prrx1 (mesenchymal cells), Pecam1 (endothelial cells), or Ptprc /CD45 (immune cells) of all cells 10 days post-fracture. (B) Top 5 DEGs for each cluster of all cells from 10 DPF. (C) UMAP colored by group (Control, Exptl). The percent of cells in each cluster by group was quantified (number of cells in cluster/total number of cells from that callus) for each replicate callus and plotted (n=3). (D) Number of Skeletal Stem and Progenitor Cells (SSPCs, defined as cells expressing Acta2 , Ly6a , Itgav , Thy1 , and Ctsk ) normalized to the total number of cells from that replicate callus. (E) Expression of myofibroblast genes ( Acta2 , Tagln2 , Actg1 , and Tpm2 ), nonspecific fibroblast genes ( Col1a1 , Col3a1 , and Postn ), and innate immune genes ( Isg15 , Ifit1 , Irf7 ) used to annotate mesenchymal cell clusters. (F) MicroCT analysis of fracture callus. (G) Histological analysis of callus composition. (Graphs depict mean±SD; statistical differences were determined by a two-Way ANOVA with Holm-Sidak Post-Hoc test.)

    Article Snippet: Femurs were scanned ex vivo using microCT (VivaCT 40, Scanco, 10.5μm voxel size, 55kV, 145μA, 300ms integration time).

    Techniques: Control, Expressing

    (A) Mice were treated with TMX starting at 10-wks age, followed by femur fracture at 12-wks, followed by 2 weeks GCV treatment. Healing was assessed 14 days post-fracture. Genotype controls were mice lacking either Osx-CreERT2 (Cre-) or ROSA-TK (TK-) alleles; experimental mice had both alleles (Cre+/TK+) (n=6). ( B) TK+ mice were treated with water for TK IHC, which shows robust TK expression in callus woven bone only in Cre+ mice (green dashed line=callus periphery; WoB=Woven Bone; Mu=Muscle). (C) (Left) Representative radiographs. (Right) Quantification of radiographic healing. ( D) Histological analysis of PSRAB stained-sections to assess callus size and composition. (E) Callus bone volume was quantified by microCT. (Statistical differences determined by Chi-Square test (C) or by two-way ANOVA with Holm-Sidak Post-Hoc test (D, E); *p<0.05, **p<0.01. Scale bars: TK=0.25 mm, PSRAB=1 mm, microCT=1 mm.)

    Journal: bioRxiv

    Article Title: Proliferation of Early and Mature Osteoblasts Is Required for Bone Fracture Healing in Mice

    doi: 10.1101/2025.05.27.656371

    Figure Lengend Snippet: (A) Mice were treated with TMX starting at 10-wks age, followed by femur fracture at 12-wks, followed by 2 weeks GCV treatment. Healing was assessed 14 days post-fracture. Genotype controls were mice lacking either Osx-CreERT2 (Cre-) or ROSA-TK (TK-) alleles; experimental mice had both alleles (Cre+/TK+) (n=6). ( B) TK+ mice were treated with water for TK IHC, which shows robust TK expression in callus woven bone only in Cre+ mice (green dashed line=callus periphery; WoB=Woven Bone; Mu=Muscle). (C) (Left) Representative radiographs. (Right) Quantification of radiographic healing. ( D) Histological analysis of PSRAB stained-sections to assess callus size and composition. (E) Callus bone volume was quantified by microCT. (Statistical differences determined by Chi-Square test (C) or by two-way ANOVA with Holm-Sidak Post-Hoc test (D, E); *p<0.05, **p<0.01. Scale bars: TK=0.25 mm, PSRAB=1 mm, microCT=1 mm.)

    Article Snippet: Femurs were scanned ex vivo using microCT (VivaCT 40, Scanco, 10.5μm voxel size, 55kV, 145μA, 300ms integration time).

    Techniques: Expressing, Staining

    (A) Histological analysis of callus. Ablation of proliferating Osterix-expressing cells for 14 days post-fracture significantly reduces the amount of woven bone and significantly increases the amount of fibrous tissue within the fracture callus, without affecting %cartilage. These data are summarized in the stacked bar graph in . (B) microCT analysis of the callus region (includes callus + cortical bone). Ablation of proliferating Osterix-expressing cells for 14 days post-fracture significantly reduces Total Volume (TV) and Bone Volume (BV) at the site of fracture callus. (Dashed lines indicate avg. value of contralateral intact femurs; shading denotes +/- SD). (C) microCT analysis of non-fractured bones. Treatment with GCV does not significantly change TV, BV, or femur length in the contralateral, non-fractured femurs. (All mice in A-C were dosed with GCV.) (D) Impairments in fracture callus size and bone volume are only seen in mice that are Cre+;TK+ and treated with GCV, even if all received TMX treatment. (E) Impairments in fracture callus size and bone volume are only seen in mice that are Cre+;TK+ and are treated with TMX, even if all received GCV treatment. (The third bar of graphs in panels D and E show identical data, also shown in the fourth bar of panel B.) (Graphs depict mean±SD and statistical differences were determined by (A-C) a Two-Way ANOVA with Holm-Sidak Post-Hoc test or (D & E) Kruskal-Wallis test.)

    Journal: bioRxiv

    Article Title: Proliferation of Early and Mature Osteoblasts Is Required for Bone Fracture Healing in Mice

    doi: 10.1101/2025.05.27.656371

    Figure Lengend Snippet: (A) Histological analysis of callus. Ablation of proliferating Osterix-expressing cells for 14 days post-fracture significantly reduces the amount of woven bone and significantly increases the amount of fibrous tissue within the fracture callus, without affecting %cartilage. These data are summarized in the stacked bar graph in . (B) microCT analysis of the callus region (includes callus + cortical bone). Ablation of proliferating Osterix-expressing cells for 14 days post-fracture significantly reduces Total Volume (TV) and Bone Volume (BV) at the site of fracture callus. (Dashed lines indicate avg. value of contralateral intact femurs; shading denotes +/- SD). (C) microCT analysis of non-fractured bones. Treatment with GCV does not significantly change TV, BV, or femur length in the contralateral, non-fractured femurs. (All mice in A-C were dosed with GCV.) (D) Impairments in fracture callus size and bone volume are only seen in mice that are Cre+;TK+ and treated with GCV, even if all received TMX treatment. (E) Impairments in fracture callus size and bone volume are only seen in mice that are Cre+;TK+ and are treated with TMX, even if all received GCV treatment. (The third bar of graphs in panels D and E show identical data, also shown in the fourth bar of panel B.) (Graphs depict mean±SD and statistical differences were determined by (A-C) a Two-Way ANOVA with Holm-Sidak Post-Hoc test or (D & E) Kruskal-Wallis test.)

    Article Snippet: Femurs were scanned ex vivo using microCT (VivaCT 40, Scanco, 10.5μm voxel size, 55kV, 145μA, 300ms integration time).

    Techniques: Expressing

    (A) Femur fracture was performed at 12-wks age, followed by 2 weeks GCV treatment. Healing was assessed 14 days after fracture. Genotype controls included mice lacking either Ocn-Cre or RosaTK alleles; experimental mice were Cre+/TK+ (n=5-10). (B) TK+ mice were treated with water for HSV-TK IHC, which shows TK expression in callus woven bone only in Cre+ mice. (C) (Left) Representative radiographs. (Right) Quantification of radiographic healing. (D) Histological analysis of fracture callus composition on PSRAB-stained slides showed that experimental mice have altered composition, with less woven bone and more fibrous tissue compared to control. (E) Callus bone volume quantified by microCT. (Statistical differences determined by Chi-Square test (C) or two-way ANOVA with Holm-Sidak Post-Hoc test (D, E). Scale bars: TK=0.25 mm, PSRAB=1 mm, microCT=1 mm.)

    Journal: bioRxiv

    Article Title: Proliferation of Early and Mature Osteoblasts Is Required for Bone Fracture Healing in Mice

    doi: 10.1101/2025.05.27.656371

    Figure Lengend Snippet: (A) Femur fracture was performed at 12-wks age, followed by 2 weeks GCV treatment. Healing was assessed 14 days after fracture. Genotype controls included mice lacking either Ocn-Cre or RosaTK alleles; experimental mice were Cre+/TK+ (n=5-10). (B) TK+ mice were treated with water for HSV-TK IHC, which shows TK expression in callus woven bone only in Cre+ mice. (C) (Left) Representative radiographs. (Right) Quantification of radiographic healing. (D) Histological analysis of fracture callus composition on PSRAB-stained slides showed that experimental mice have altered composition, with less woven bone and more fibrous tissue compared to control. (E) Callus bone volume quantified by microCT. (Statistical differences determined by Chi-Square test (C) or two-way ANOVA with Holm-Sidak Post-Hoc test (D, E). Scale bars: TK=0.25 mm, PSRAB=1 mm, microCT=1 mm.)

    Article Snippet: Femurs were scanned ex vivo using microCT (VivaCT 40, Scanco, 10.5μm voxel size, 55kV, 145μA, 300ms integration time).

    Techniques: Expressing, Staining, Control

    (A) Fracture callus composition was determined from histology. TK+/Ocn-Cre+ mice have reduced woven bone and increased fibrous tissue within the fracture callus without affecting cartilage composition. These data are summarized in the stacked bar graph in . (B) MicroCT analysis of callus region (includes callus + cortical bone). TK+/OcnCre+ mice have reduced Total Volume (TV) and Bone Volume (BV). (Dashed lines indicate avg. value of contralateral intact femurs; shading denotes +/- SD). (C) MicroCT of intact femurs. Genotype does not alter TV, BV, or Femur Length in the contralateral, non-fractured femurs from the same cohort of mice. (All mice in A-C were dosed with GCV.) (D) Impairments in fracture callus morphology are only seen in mice that are Cre+;TK+ and treated with GCV. (Graphs depict mean±SD and statistical differences were determined by a two-Way ANOVA with Holm-Sidak Post-Hoc test.)

    Journal: bioRxiv

    Article Title: Proliferation of Early and Mature Osteoblasts Is Required for Bone Fracture Healing in Mice

    doi: 10.1101/2025.05.27.656371

    Figure Lengend Snippet: (A) Fracture callus composition was determined from histology. TK+/Ocn-Cre+ mice have reduced woven bone and increased fibrous tissue within the fracture callus without affecting cartilage composition. These data are summarized in the stacked bar graph in . (B) MicroCT analysis of callus region (includes callus + cortical bone). TK+/OcnCre+ mice have reduced Total Volume (TV) and Bone Volume (BV). (Dashed lines indicate avg. value of contralateral intact femurs; shading denotes +/- SD). (C) MicroCT of intact femurs. Genotype does not alter TV, BV, or Femur Length in the contralateral, non-fractured femurs from the same cohort of mice. (All mice in A-C were dosed with GCV.) (D) Impairments in fracture callus morphology are only seen in mice that are Cre+;TK+ and treated with GCV. (Graphs depict mean±SD and statistical differences were determined by a two-Way ANOVA with Holm-Sidak Post-Hoc test.)

    Article Snippet: Femurs were scanned ex vivo using microCT (VivaCT 40, Scanco, 10.5μm voxel size, 55kV, 145μA, 300ms integration time).

    Techniques:

    (A) Ocn-Cre;ROSA-TK mice were treated with GCV for 2 weeks post-fracture (as depicted in ), then GCV was withdrawn for the following 10 weeks. X-ray scoring shows that Ocn-Cre-mice fully bridge and remodel by 12 weeks post-fracture, whereas Ocn-Cre+ mice have significantly impaired bridging at 2 weeks post-fracture that does not fully recover by 12 weeks. (B) PSRAB staining of representative samples shows that Ocn-Cre-mice 12 weeks post-fracture have bony healing at the fracture site, whereas Ocn-Cre+ mice still have cartilaginous and fibrotic calluses 12 weeks post-fracture. (C) Ocn-Cre;ROSA-TK mice were treated with GCV for 3-, 5-, or 10-days post-fracture and euthanized on the last day of treatment indicated. PSRAB staining and analysis shows early changes in callus composition. (D) microCT analysis of Ocn-Cre;RosaTK mice treated with GCV for 3-, 5-, or 10-days post-fracture and euthanized on the last day of treatment indicated shows that control Cre-mice increase callus mineralization over time, whereas Cre+ experimental mice do not create a mineralized callus. (Graphs depict mean±SD; statistical differences were determined by (A) Chi-Square test, or (C-D) two-Way ANOVA with Holm-Sidak Post-Hoc test.)

    Journal: bioRxiv

    Article Title: Proliferation of Early and Mature Osteoblasts Is Required for Bone Fracture Healing in Mice

    doi: 10.1101/2025.05.27.656371

    Figure Lengend Snippet: (A) Ocn-Cre;ROSA-TK mice were treated with GCV for 2 weeks post-fracture (as depicted in ), then GCV was withdrawn for the following 10 weeks. X-ray scoring shows that Ocn-Cre-mice fully bridge and remodel by 12 weeks post-fracture, whereas Ocn-Cre+ mice have significantly impaired bridging at 2 weeks post-fracture that does not fully recover by 12 weeks. (B) PSRAB staining of representative samples shows that Ocn-Cre-mice 12 weeks post-fracture have bony healing at the fracture site, whereas Ocn-Cre+ mice still have cartilaginous and fibrotic calluses 12 weeks post-fracture. (C) Ocn-Cre;ROSA-TK mice were treated with GCV for 3-, 5-, or 10-days post-fracture and euthanized on the last day of treatment indicated. PSRAB staining and analysis shows early changes in callus composition. (D) microCT analysis of Ocn-Cre;RosaTK mice treated with GCV for 3-, 5-, or 10-days post-fracture and euthanized on the last day of treatment indicated shows that control Cre-mice increase callus mineralization over time, whereas Cre+ experimental mice do not create a mineralized callus. (Graphs depict mean±SD; statistical differences were determined by (A) Chi-Square test, or (C-D) two-Way ANOVA with Holm-Sidak Post-Hoc test.)

    Article Snippet: Femurs were scanned ex vivo using microCT (VivaCT 40, Scanco, 10.5μm voxel size, 55kV, 145μA, 300ms integration time).

    Techniques: Staining, Control

    (A) Mice were treated with TMX starting at 10-wks age, followed by femur fracture at 12-wks, followed by 2 weeks GCV treatment. Healing was assessed 14 days after fracture. Control mice were Cre-;TK+ while experimental mice were Cre+;TK+. (B) TK+ mice were treated with water for HSV-TK IHC, which shows robust TK expression in callus woven bone only in Cre+ mice. (C) Fracture callus bridging was quantified based on radiographic images. (D) Histological analysis of PSRAB stained-sections was used to assess callus size and composition. (E) Callus bone volume was quantified using microCT. (Statistical differences were determined by Chi-Square test (B) or by two-tailed t-test (C, D). Scale bars: PSRAB=1 mm, TK IHC=0.25 mm, microCT=1 mm.)

    Journal: bioRxiv

    Article Title: Proliferation of Early and Mature Osteoblasts Is Required for Bone Fracture Healing in Mice

    doi: 10.1101/2025.05.27.656371

    Figure Lengend Snippet: (A) Mice were treated with TMX starting at 10-wks age, followed by femur fracture at 12-wks, followed by 2 weeks GCV treatment. Healing was assessed 14 days after fracture. Control mice were Cre-;TK+ while experimental mice were Cre+;TK+. (B) TK+ mice were treated with water for HSV-TK IHC, which shows robust TK expression in callus woven bone only in Cre+ mice. (C) Fracture callus bridging was quantified based on radiographic images. (D) Histological analysis of PSRAB stained-sections was used to assess callus size and composition. (E) Callus bone volume was quantified using microCT. (Statistical differences were determined by Chi-Square test (B) or by two-tailed t-test (C, D). Scale bars: PSRAB=1 mm, TK IHC=0.25 mm, microCT=1 mm.)

    Article Snippet: Femurs were scanned ex vivo using microCT (VivaCT 40, Scanco, 10.5μm voxel size, 55kV, 145μA, 300ms integration time).

    Techniques: Control, Expressing, Staining, Two Tailed Test

    (A) Cre;ROSA-TK mice were treated with GCV and also treated with carbenoxolone (CBX) to block gap junction intercellular communication. In three lines of mice receiving CBX, callus bone volume measured by microCT was significantly less in Cre+ experimental vs. Cre-control. Callus bone volume in Cre+/CBX+ mice was not different from experimental Cre+ mice that did not receive CBX (the latter data also shown in , , and ). (B) When data from each Cre line were aggregated to overcome low sample size, Cre+ mice had impaired callus mineralization compared to Cre-mice, both treated with CBX. (Graphs depict mean±SD; statistical differences were determined by One-Way ANOVA with Fisher’s LSD Post-Hoc (A) or Mann-Whitney U test (B).)

    Journal: bioRxiv

    Article Title: Proliferation of Early and Mature Osteoblasts Is Required for Bone Fracture Healing in Mice

    doi: 10.1101/2025.05.27.656371

    Figure Lengend Snippet: (A) Cre;ROSA-TK mice were treated with GCV and also treated with carbenoxolone (CBX) to block gap junction intercellular communication. In three lines of mice receiving CBX, callus bone volume measured by microCT was significantly less in Cre+ experimental vs. Cre-control. Callus bone volume in Cre+/CBX+ mice was not different from experimental Cre+ mice that did not receive CBX (the latter data also shown in , , and ). (B) When data from each Cre line were aggregated to overcome low sample size, Cre+ mice had impaired callus mineralization compared to Cre-mice, both treated with CBX. (Graphs depict mean±SD; statistical differences were determined by One-Way ANOVA with Fisher’s LSD Post-Hoc (A) or Mann-Whitney U test (B).)

    Article Snippet: Femurs were scanned ex vivo using microCT (VivaCT 40, Scanco, 10.5μm voxel size, 55kV, 145μA, 300ms integration time).

    Techniques: Blocking Assay, Control, MANN-WHITNEY

    (A) Mice were treated with TMX starting at 10-wks age, followed by femur fracture at 12-wks, followed by 2 weeks GCV treatment. Healing was assessed 14 days after fracture. Control mice were Cre-;TK+ while experimental mice were Cre+;TK+. (B) Radiographs showed less mineralized callus in Cre+ mice, resulting in modestly poorer callus bridging scores than in Cre-controls. (C) Histological analysis shows a marginally smaller callus area in Cre+ mice, but normal percent cartilage and woven bone compared to Cre-controls. (D) Fractured bones were assessed by microCT; there were no significant differences in callus bone volume or BMD between Cre+ mice and control mice. (Statistical differences were determined by Chi-Square test (B) or by two-tailed t-test (C,D). Scale bars: PSRAB=1 mm, TK IHC=0.5 mm, microCT=1 mm.)

    Journal: bioRxiv

    Article Title: Proliferation of Early and Mature Osteoblasts Is Required for Bone Fracture Healing in Mice

    doi: 10.1101/2025.05.27.656371

    Figure Lengend Snippet: (A) Mice were treated with TMX starting at 10-wks age, followed by femur fracture at 12-wks, followed by 2 weeks GCV treatment. Healing was assessed 14 days after fracture. Control mice were Cre-;TK+ while experimental mice were Cre+;TK+. (B) Radiographs showed less mineralized callus in Cre+ mice, resulting in modestly poorer callus bridging scores than in Cre-controls. (C) Histological analysis shows a marginally smaller callus area in Cre+ mice, but normal percent cartilage and woven bone compared to Cre-controls. (D) Fractured bones were assessed by microCT; there were no significant differences in callus bone volume or BMD between Cre+ mice and control mice. (Statistical differences were determined by Chi-Square test (B) or by two-tailed t-test (C,D). Scale bars: PSRAB=1 mm, TK IHC=0.5 mm, microCT=1 mm.)

    Article Snippet: Femurs were scanned ex vivo using microCT (VivaCT 40, Scanco, 10.5μm voxel size, 55kV, 145μA, 300ms integration time).

    Techniques: Control, Two Tailed Test

    Twelve-week old male Cre-negative (Cre-) and Cre-positive (Cre +) Bmpr1a fl/fl ;LysM-Cre mice remained euthyroid (CO) or were rendered hyperthyroid (T4) by adding 1.2 µg/mL L-thyroxine into their drinking water over 4 weeks. Using microCT analysis, ( a ) bone volume per total volume (BV/TV), ( b ) trabecular number (Tb.N), ( c ) trabecular separation (Tb.Sp), and ( d ) trabecular thickness (Tb.Th) were determined at the fourth lumbar vertebra (L4). e Representative 3D reconstructions of the trabecular compartment of L4. Additionally, ( f ) trabecular BV/TV and ( g ) cortical bone volume over total volume (Ct.BV/TV) were assessed at the femur. The maximal force (F max ) as an indicator of bone strength ( h ) at the L5 vertebra and ( i ) femur was tested by compression test and 3-point bending test, respectively. Serum concentrations of ( j ) bone formation marker P1NP and ( k ) bone resorption marker TRAP were analyzed by ELISA. Furthermore, ( l ) the bone formation rate per bone surface (BFR) and ( m ) osteoclast surface per bone surface (Oc.S/BS) were assessed at the L3 and L4 vertebra, respectively. In vitro, ( n ) expression of acid phosphatase 5, tartrate resistant ( Acp5 ), encoding for TRAP, in primary osteoclasts derived from Bmpr1a fl/fl ;LysM-Cre mice at day 7 of differentiation, with or w/o treatment with 100 nM T 3 (T3) over 48 h, was quantified using quantitative real-time PCR. Each dot indicates an individual mouse. In vivo: Cre-/CO: N = 9; Cre-/T4: N = 8; Cre +/CO: N = 9; Cre + /T4: N = 9; In vitro: Cre-/CO: N = 5; Cre-/T3: N = 6; Cre +/CO: N = 6; Cre +/T3: N = 6. The horizontal lines represent the mean +/- SD. Statistical analysis was performed by Two-way ANOVA and selected p values are shown within the graph.

    Journal: Communications Biology

    Article Title: Hyperthyroidism-driven bone loss depends on BMP receptor Bmpr1a expression in osteoblasts

    doi: 10.1038/s42003-024-06227-0

    Figure Lengend Snippet: Twelve-week old male Cre-negative (Cre-) and Cre-positive (Cre +) Bmpr1a fl/fl ;LysM-Cre mice remained euthyroid (CO) or were rendered hyperthyroid (T4) by adding 1.2 µg/mL L-thyroxine into their drinking water over 4 weeks. Using microCT analysis, ( a ) bone volume per total volume (BV/TV), ( b ) trabecular number (Tb.N), ( c ) trabecular separation (Tb.Sp), and ( d ) trabecular thickness (Tb.Th) were determined at the fourth lumbar vertebra (L4). e Representative 3D reconstructions of the trabecular compartment of L4. Additionally, ( f ) trabecular BV/TV and ( g ) cortical bone volume over total volume (Ct.BV/TV) were assessed at the femur. The maximal force (F max ) as an indicator of bone strength ( h ) at the L5 vertebra and ( i ) femur was tested by compression test and 3-point bending test, respectively. Serum concentrations of ( j ) bone formation marker P1NP and ( k ) bone resorption marker TRAP were analyzed by ELISA. Furthermore, ( l ) the bone formation rate per bone surface (BFR) and ( m ) osteoclast surface per bone surface (Oc.S/BS) were assessed at the L3 and L4 vertebra, respectively. In vitro, ( n ) expression of acid phosphatase 5, tartrate resistant ( Acp5 ), encoding for TRAP, in primary osteoclasts derived from Bmpr1a fl/fl ;LysM-Cre mice at day 7 of differentiation, with or w/o treatment with 100 nM T 3 (T3) over 48 h, was quantified using quantitative real-time PCR. Each dot indicates an individual mouse. In vivo: Cre-/CO: N = 9; Cre-/T4: N = 8; Cre +/CO: N = 9; Cre + /T4: N = 9; In vitro: Cre-/CO: N = 5; Cre-/T3: N = 6; Cre +/CO: N = 6; Cre +/T3: N = 6. The horizontal lines represent the mean +/- SD. Statistical analysis was performed by Two-way ANOVA and selected p values are shown within the graph.

    Article Snippet: Also, femurs and L4 were analyzed using micro-computed tomography (microCT) (vivaCT 40, Scanco Medical, Brüttisellen, Switzerland) with an X-ray energy of 70 kVp and isotropic voxel size of 10.5 μm (114 mA, 200 msec integration time).

    Techniques: Marker, Enzyme-linked Immunosorbent Assay, In Vitro, Expressing, Derivative Assay, Real-time Polymerase Chain Reaction, In Vivo

    Twelve-week old male Cre-negative (Cre-) and Cre-positive (Cre +) Bmpr1a fl/fl ;Osx-Cre mice remained euthyroid (CO) or were rendered hyperthyroid (T4) by adding 1.2 µg/mL L-thyroxine into their drinking water over 4 weeks. Based on microCT analysis, ( a ) bone volume over total volume (BV/TV), ( b ) trabecular number (Tb.N), ( c ) trabecular thickness (Tb.Th), and ( d ) trabecular separation (Tb.Sp) were measured at the fourth lumbar vertebra (L4). e The maximal force (F max ) was assessed by compression test at the L5 vertebra. f Representative 3D reconstructions of the trabecular compartment of L4. In addition, ( g ) trabecular BV/TV and ( h ) cortical bone volume over total volume (Ct.BV/TV), ( i ) Ct. thickness (Ct.Th) and ( j ) cortical bone mineral density (Ct.BMD) were determined at the femur. k The maximal force (F max ) at the femur was tested by 3-point bending test. l Serum concentrations of bone formation marker P1NP was quantified using ELISA. Further, ( m ) osteoblast surface per bone surface (Ob.S/BS) and ( n ) bone formation rate per bone surface (BFR) were assessed at the spine. With regards to bone resorption, serum concentrations of ( o ) bone resorption marker TRAP and ( p ) osteoclast surface per bone surface (Oc.S/BS) were determined. Each dot indicates an individual mouse. MicroCT/Histology: Cre-/CO: N = 9; Cre-/T4: N = 9; Cre +/CO: N = 8; Cre +/T4: N = 9; ELISAs: Cre-/CO: N = 7; Cre-/T4: N = 8; Cre +/CO: N = 7; Cre +/T4: N = 8. The horizontal lines represent the mean +/- SD. Statistical analysis was performed by Two-way ANOVA and selected p values are shown within the graph.

    Journal: Communications Biology

    Article Title: Hyperthyroidism-driven bone loss depends on BMP receptor Bmpr1a expression in osteoblasts

    doi: 10.1038/s42003-024-06227-0

    Figure Lengend Snippet: Twelve-week old male Cre-negative (Cre-) and Cre-positive (Cre +) Bmpr1a fl/fl ;Osx-Cre mice remained euthyroid (CO) or were rendered hyperthyroid (T4) by adding 1.2 µg/mL L-thyroxine into their drinking water over 4 weeks. Based on microCT analysis, ( a ) bone volume over total volume (BV/TV), ( b ) trabecular number (Tb.N), ( c ) trabecular thickness (Tb.Th), and ( d ) trabecular separation (Tb.Sp) were measured at the fourth lumbar vertebra (L4). e The maximal force (F max ) was assessed by compression test at the L5 vertebra. f Representative 3D reconstructions of the trabecular compartment of L4. In addition, ( g ) trabecular BV/TV and ( h ) cortical bone volume over total volume (Ct.BV/TV), ( i ) Ct. thickness (Ct.Th) and ( j ) cortical bone mineral density (Ct.BMD) were determined at the femur. k The maximal force (F max ) at the femur was tested by 3-point bending test. l Serum concentrations of bone formation marker P1NP was quantified using ELISA. Further, ( m ) osteoblast surface per bone surface (Ob.S/BS) and ( n ) bone formation rate per bone surface (BFR) were assessed at the spine. With regards to bone resorption, serum concentrations of ( o ) bone resorption marker TRAP and ( p ) osteoclast surface per bone surface (Oc.S/BS) were determined. Each dot indicates an individual mouse. MicroCT/Histology: Cre-/CO: N = 9; Cre-/T4: N = 9; Cre +/CO: N = 8; Cre +/T4: N = 9; ELISAs: Cre-/CO: N = 7; Cre-/T4: N = 8; Cre +/CO: N = 7; Cre +/T4: N = 8. The horizontal lines represent the mean +/- SD. Statistical analysis was performed by Two-way ANOVA and selected p values are shown within the graph.

    Article Snippet: Also, femurs and L4 were analyzed using micro-computed tomography (microCT) (vivaCT 40, Scanco Medical, Brüttisellen, Switzerland) with an X-ray energy of 70 kVp and isotropic voxel size of 10.5 μm (114 mA, 200 msec integration time).

    Techniques: Marker, Enzyme-linked Immunosorbent Assay